Validation Data Gallery
Filter:
Tested Applications
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24371-1-AP targets TRIM61 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRIM61 fusion protein Ag21656 相同性解析による交差性が予測される生物種 |
| Full Name | tripartite motif-containing 61 |
| Calculated molecular weight | 209 aa, 24 kDa |
| GenBank accession number | BC089393 |
| Gene Symbol | TRIM61 |
| Gene ID (NCBI) | 391712 |
| RRID | AB_3669434 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5EBN2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRIM61 is a member of the tripartite motif (TRIM) protein family. The RING domain in TRIM proteins, including TRIM61, can mediate the conjugation of proteins with ubiquitin, small ubiquitin-like modifier (SUMO), or the ubiquitin-like molecule IFN-stimulated protein of 15 kDa (ISG15), contributing to the biological flexibility of TRIM proteins. The E3 ligase activity of TRIM proteins, such as TRIM61, is essential for their role in post-translational modification, which plays a crucial role in cellular processes often resulting in targeted degradation of conjugated proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRIM61 antibody 24371-1-AP | Download protocol |
| IF protocol for TRIM61 antibody 24371-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |