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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM61 Polyclonal antibody

TRIM61 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24371-1-AP

Synonyms

tripartite motif containing 61, TRIM, RNF35, RING finger protein 35

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse liver tissue using TRIM61 Polyclonal antibody (24371-1-AP)

    IHC staining of mouse liver using 24371-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24371-1-AP (TRIM61 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TRIM61 Polyclonal antibody (24371-1-AP)

    IF Staining of U2OS using 24371-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM61 antibody (24371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24371-1-AP targets TRIM61 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM61 fusion protein Ag21656 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 61
Calculated molecular weight 209 aa, 24 kDa
GenBank accession numberBC089393
Gene symbol TRIM61
Gene ID (NCBI) 391712
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM61 is a member of the tripartite motif (TRIM) protein family. The RING domain in TRIM proteins, including TRIM61, can mediate the conjugation of proteins with ubiquitin, small ubiquitin-like modifier (SUMO), or the ubiquitin-like molecule IFN-stimulated protein of 15 kDa (ISG15), contributing to the biological flexibility of TRIM proteins. The E3 ligase activity of TRIM proteins, such as TRIM61, is essential for their role in post-translational modification, which plays a crucial role in cellular processes often resulting in targeted degradation of conjugated proteins.

Protocols

Product Specific Protocols
IHC protocol for TRIM61 antibody 24371-1-APDownload protocol
IF protocol for TRIM61 antibody 24371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols