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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM62 Polyclonal antibody

TRIM62 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15256-1-AP

Synonyms

Tripartite motif-containing protein 62, tripartite motif containing 62, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM62

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM62 Polyclonal antibody (15256-1-AP)

    WB analysis using 15256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15256-1-AP (TRIM62 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TRIM62 Polyclonal antibody (15256-1-AP)

    IHC staining of mouse liver using 15256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TRIM62 Polyclonal antibody (15256-1-AP)

    IHC staining of mouse liver using 15256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using TRIM62 Polyclonal antibody (15256-1-AP)

    IHC staining of rat ovary using 15256-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using TRIM62 Polyclonal antibody (15256-1-AP)

    IHC staining of rat ovary using 15256-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TRIM62 Polyclonal antibody (15256-1-AP)

    IF Staining of U2OS using 15256-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM62 antibody (15256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inBGC-823 cells, SGC-7901 cells, mouse ovary tissue
Positive IHC detected inmouse liver tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15256-1-AP targets TRIM62 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM62 fusion protein Ag7143 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 62
Calculated molecular weight 54 kDa
Observed molecular weight54 kDa
GenBank accession numberBC001222
Gene symbol TRIM62
Gene ID (NCBI) 55223
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).

Protocols

Product Specific Protocols
WB protocol for TRIM62 antibody 15256-1-APDownload protocol
IHC protocol for TRIM62 antibody 15256-1-APDownload protocol
IF protocol for TRIM62 antibody 15256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.

Authors - Uchil Pradeep D PD
View Article