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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIM62 Polyclonal antibody

TRIM62 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15256-1-AP

Synonyms

Tripartite motif-containing protein 62, tripartite motif containing 62, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM62

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM62 Polyclonal antibody (15256-1-AP)

    WB analysis using 15256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15256-1-AP (TRIM62 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TRIM62 Polyclonal antibody (15256-1-AP)

    IHC staining of mouse liver using 15256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TRIM62 Polyclonal antibody (15256-1-AP)

    IHC staining of mouse liver using 15256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using TRIM62 Polyclonal antibody (15256-1-AP)

    IHC staining of rat ovary using 15256-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat ovary tissue using TRIM62 Polyclonal antibody (15256-1-AP)

    IHC staining of rat ovary using 15256-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TRIM62 Polyclonal antibody (15256-1-AP)

    IF Staining of U2OS using 15256-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM62 antibody (15256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inBGC-823 cells, SGC-7901 cells, mouse ovary tissue
Positive IHC detected inmouse liver tissue, rat ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15256-1-AP targets TRIM62 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM62 fusion protein Ag7143 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 62
Calculated molecular weight 54 kDa
Observed molecular weight54 kDa
GenBank accession numberBC001222
Gene symbol TRIM62
Gene ID (NCBI) 55223
RRIDAB_3669202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).

Protocols

Product Specific Protocols
WB protocol for TRIM62 antibody 15256-1-APDownload protocol
IHC protocol for TRIM62 antibody 15256-1-APDownload protocol
IF protocol for TRIM62 antibody 15256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.

Authors - Uchil Pradeep D PD
View Article