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- KD/KO Validated
TRIM72 Polyclonal antibody
TRIM72 Polyclonal Antibody for WB, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF-P, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 22151-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
22151-1-AP targets TRIM72 in WB, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRIM72 fusion protein Ag17756 相同性解析による交差性が予測される生物種 |
| Full Name | tripartite motif-containing 72 |
| Calculated molecular weight | 477 aa, 53 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC033211 |
| Gene symbol | TRIM72 |
| Gene ID (NCBI) | 493829 |
| RRID | AB_2879009 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM72 antibody 22151-1-AP | Download protocol |
| IF protocol for TRIM72 antibody 22151-1-AP | Download protocol |
| IP protocol for TRIM72 antibody 22151-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice | ||
Cancer Biomark Ubiquitination and degradation of MGMT by TRIM72 increases the sensitivity of uveal melanoma cells to Dacarbazine treatment
| ||
Biochem Biophys Res Commun Mesenchymal stem cells-derived exosomes prevent sepsis-induced myocardial injury by a CircRTN4/miR-497-5p/MG53 pathway. | ||
BMC Cardiovasc Disord Rosuvastatin exerts cardioprotective effect in lipopolysaccharide-mediated injury of cardiomyocytes in an MG53-dependent manner. | ||
FASEB J Chorionic villus-derived mesenchymal stem cells induce E3 ligase TRIM72 expression and regulate cell behaviors through ubiquitination of p53 in trophoblasts
| ||
Biomol Biomed Integrated transcriptomic and proteomic analysis reveals potential targets for heart regeneration |
