Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, A549 cells, HCT 116 cells, MCF-7 cells, NIH/3T3 cells, PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85169-1-RR targets TRIM8 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | TRIM8 fusion protein Ag26517 相同性解析による交差性が予測される生物種 |
| Full Name | tripartite motif-containing 8 |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 61 kDa |
| GenBank accession number | BC021925 |
| Gene Symbol | TRIM8 |
| Gene ID (NCBI) | 81603 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9BZR9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRIM8 (Tripartite motif containing protein 8), also known as RNF27 (RING finger protein 27), a member of TRIM family proteins, is known to play a dual role as both tumor suppressor and oncogene, and to function at the crosstalk of cancer and innate immunity. TRIM8 can function at two subcellular sites-nucleus and cytoplasm-to regulate NF-κB, one of the central signaling pathways that plays a critical role in carcinogenesis and inflammatory diseases (PMID: 33230447, 22262183). TRIM8 is composed of 551 amino acids (aa) with a molecular weight of 61.5 kDa, contains a Nuclear Localization Signal (NLS), and forms specific nuclear structures similar to the TRIM19/PML Nuclear Bodies (NBs) (PMID: 33807506, 29182544).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIM8 antibody 85169-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |