Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 8 publications below |
Product Information
16124-1-AP targets TRIOBP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRIOBP fusion protein Ag9092 相同性解析による交差性が予測される生物種 |
| Full Name | TRIO and F-actin binding protein |
| Calculated molecular weight | 2356 aa, 261 kDa |
| Observed molecular weight | 68-75 kDa |
| GenBank accession number | BC013278 |
| Gene Symbol | TRIOBP |
| Gene ID (NCBI) | 11078 |
| RRID | AB_2209237 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H2D6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIOBP antibody 16124-1-AP | Download protocol |
| IHC protocol for TRIOBP antibody 16124-1-AP | Download protocol |
| IF protocol for TRIOBP antibody 16124-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Unbalanced bidirectional radial stiffness gradients within the organ of Corti promoted by TRIOBP. | ||
Mol Neurobiol Essential Role of Sptan1 in Cochlear Hair Cell Morphology and Function Via Focal Adhesion Signaling. | ||
J Cell Sci GIPC3 couples to MYO6 and PDZ domain proteins and shapes the hair cell apical region | ||
Cell Mol Life Sci TRIOBP modulates β-catenin signaling by regulation of miR-29b in idiopathic pulmonary fibrosis |