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  • Featured Product
  • KD/KO Validated

TRIP10 Polyclonal antibody

TRIP10 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10798-1-AP

Synonyms

TRIP-10, TR-interacting protein 10, Thyroid receptor-interacting protein 10, hSTP, CIP4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIP10 Polyclonal antibody (10798-1-AP)

    WB analysis using 10798-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TRIP10 Polyclonal antibody (10798-1-AP)

    WB analysis of K-562 using 10798-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using TRIP10 Polyclonal antibody (10798-1-AP)

    WB analysis of BxPC-3 using 10798-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TRIP10 Polyclonal antibody (10798-1-AP)

    WB analysis of mouse skeletal muscle using 10798-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using TRIP10 Polyclonal antibody (10798-1-AP)

    IP experiment of K-562 using 10798-1-AP

    IP result of anti-TRIP10 (IP:10798-1-AP, 4ug; Detection:10798-1-AP 1:10000) with K-562 cells lysate 1760 ug.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using TRIP10 Polyclonal antibody (10798-1-AP)

    IF Staining of BxPC-3 using 10798-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 10798-1-AP (TRIP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRIP10 Polyclonal antibody (10798-1-AP)

    IF Staining of HeLa using 10798-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIP10 antibody (10798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRIP10 Polyclonal antibody (10798-1-AP)

    IF Staining of HeLa using 10798-1-AP

    Immunofluorescent analysis of HeLa cells using 10798-1-AP (TRIP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, BxPC-3 cells, mouse skeletal muscle tissue, HeLa cells, NIH/3T3 cells, C6 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inHeLa cells, BxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10798-1-AP targets TRIP10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIP10 fusion protein Ag1251 相同性解析による交差性が予測される生物種
Full Name thyroid hormone receptor interactor 10
Calculated molecular weight 63 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC013002
Gene symbol TRIP10
Gene ID (NCBI) 9322
RRIDAB_2209248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.

Protocols

Product Specific Protocols
WB protocol for TRIP10 antibody 10798-1-APDownload protocol
IF protocol for TRIP10 antibody 10798-1-APDownload protocol
IP protocol for TRIP10 antibody 10798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins.

Authors - Shinya Yuge
View Article
humanWB,IHC

J Exp Clin Cancer Res

CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling.

Authors - Dong-Fang Meng
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

anwesh (Verified Customer) (04-01-2019)

Great antibody. Detects human version of the protein in several cell types. 293T, U2OS and A549 cells tested. Antibody also IP's the endogenous protein

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T
TRIP10 Antibody Western Blot,Immunoprecipitation, validation (1:1000 dilution) in 293T (Cat no:10798-1-AP)