Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis using 10798-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10798-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 10798-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 10798-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10798-1-AP (TRIP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 10798-1-AP
IP result of anti-TRIP10 (IP:10798-1-AP, 4ug; Detection:10798-1-AP 1:10000) with K-562 cells lysate 1760 ug.
IF Staining of BxPC-3 using 10798-1-AP
Immunofluorescent analysis of BxPC-3 cells using 10798-1-AP (TRIP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10798-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIP10 antibody (10798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 10798-1-AP
Immunofluorescent analysis of HeLa cells using 10798-1-AP (TRIP10 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

TRIP10 Polyclonal antibody

TRIP10 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 10798-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TRIP-10, TR-interacting protein 10, Thyroid receptor-interacting protein 10, hSTP, CIP4

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration: 150 μL, 900 ug/ml

20 μL, 900 ug/ml

150 μL, 900 ug/ml

¥67,000/ 150 μL

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, K-562 cells, BxPC-3 cells, mouse skeletal muscle tissue, HeLa cells, NIH/3T3 cells, C6 cells
Positive IP detected in	K-562 cells
Positive IF/ICC detected in	HeLa cells, BxPC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

10798-1-AP targets TRIP10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRIP10 fusion protein Ag1251 相同性解析による交差性が予測される生物種
Full Name	thyroid hormone receptor interactor 10
Calculated molecular weight	63 kDa
Observed molecular weight	75-85 kDa
GenBank accession number	BC013002
Gene Symbol	TRIP10
Gene ID (NCBI)	9322
RRID	AB_2209248
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15642
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.

Protocols

Product Specific Protocols
WB protocol for TRIP10 antibody 10798-1-AP	Download protocol
IF protocol for TRIP10 antibody 10798-1-AP	Download protocol
IP protocol for TRIP10 antibody 10798-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nat Commun Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins. Authors - Shinya Yuge View Article
human	WB,IHC	J Exp Clin Cancer Res CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling. Authors - Dong-Fang Meng KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10798-1-AP

WB analysis of K-562 using 10798-1-AP

WB analysis of BxPC-3 using 10798-1-AP

WB analysis of mouse skeletal muscle using 10798-1-AP

IP experiment of K-562 using 10798-1-AP

IF Staining of BxPC-3 using 10798-1-AP

IF Staining of HeLa using 10798-1-AP

IF Staining of HeLa using 10798-1-AP

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