Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
25303-1-AP targets TRIP12 in WB, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRIP12 fusion protein Ag18112 相同性解析による交差性が予測される生物種 |
| Full Name | thyroid hormone receptor interactor 12 |
| Calculated molecular weight | 1992 aa, 220 kDa |
| Observed molecular weight | 190-220 kDa |
| GenBank accession number | BC114556 |
| Gene Symbol | TRIP12 |
| Gene ID (NCBI) | 9320 |
| RRID | AB_2880020 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14669 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIP12 antibody 25303-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.
| ||
Clin Epigenetics EZH2 K63-polyubiquitination affecting migration in extranodal natural killer/T-cell lymphoma
| ||
Oncogene Engineered targeting OIP5 sensitizes bladder cancer to chemotherapy resistance via TRIP12-PPP1CB-YBX1 axis | ||