TRIP4 Polyclonal antibody

Name: TRIP4 Polyclonal antibody
Brand: Proteintech
SKU: 12324-1-AP

TRIP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12324-1-AP

Synonyms

ASC 1, HsT17391, TR interacting protein 4, TRIP 4, TRIP4

Validation Data Gallery

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AllWBIHC

WB analysis of HL-60 using 12324-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12324-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12324-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12324-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 12324-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 12324-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12324-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12324-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12324-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 12324-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HL-60 cells, COLO 320 cells, HeLa cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

12324-1-AP targets TRIP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRIP4 fusion protein Ag2987 相同性解析による交差性が予測される生物種
Full Name	thyroid hormone receptor interactor 4
Calculated molecular weight	581 aa, 66 kDa
Observed molecular weight	60-65 kDa
GenBank accession number	BC012448
Gene symbol	TRIP4
Gene ID (NCBI)	9325
RRID	AB_10646482
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Thyroid hormone receptor interactor 4(TRIP4), other named ASC1, is a transcriptional coactivator that promote transcriptional efficiencies. It harbors an autonomous transactivation domain that contains a putative zinc finger motif, which serves as a binding site for nuclear receptors for thyroid, estrogen, all-trans-retinoic acid and RXR by interaction with SRC-1 or CBP/p300. There are two forms of ASC1 have been detected in mRNA level, isoform1(581aa, ~65kd) and isoform2(539aa,~60kd)(12077347,12390891)

Protocols

Product Specific Protocols
WB protocol for TRIP4 antibody 12324-1-AP	Download protocol
IHC protocol for TRIP4 antibody 12324-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Genet Med A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy. Authors - Min Ni View Article

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研究分野

Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIP4 Polyclonal antibody

TRIP4 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HL-60 using 12324-1-AP

WB analysis of HeLa using 12324-1-AP

WB analysis of COLO 320 using 12324-1-AP

WB analysis of HeLa using 12324-1-AP

WB analysis of HL-60 using 12324-1-AP

IHC staining of human liver cancer using 12324-1-AP

IHC staining of human liver cancer using 12324-1-AP

IHC staining of human liver cancer using 12324-1-AP

IHC staining of human liver cancer using 12324-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy.