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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRIP6 Polyclonal antibody

TRIP6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21163-1-AP

Synonyms

OIP 1, OIP1, Opa interacting protein 1, TR interacting protein 6, TRIP 6, TRIP6, ZRP 1, Zyxin related protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TRIP6 Polyclonal antibody (21163-1-AP)

    WB analysis of HeLa using 21163-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21163-1-AP (TRIP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using TRIP6 Polyclonal antibody (21163-1-AP)

    WB analysis of mouse lung using 21163-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21163-1-AP (TRIP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using TRIP6 Polyclonal antibody (21163-1-AP)

    WB analysis of human testis using 21163-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 21163-1-AP (TRIP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TRIP6 Polyclonal antibody (21163-1-AP)

    WB analysis of HepG2 using 21163-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21163-1-AP (TRIP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TRIP6 Polyclonal antibody (21163-1-AP)

    IF Staining of HepG2 using 21163-1-AP

    Immunofluorescent analysis of HepG2 cells, using TRIP6 antibody 21163-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human testis tissue, mouse lung tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21163-1-AP is TRIP6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIP6 fusion protein Ag15149 相同性解析による交差性が予測される生物種
Full Name thyroid hormone receptor interactor 6
Calculated molecular weight 476 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC004249
Gene symbol TRIP6
Gene ID (NCBI) 7205
RRIDAB_10732813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIP6 (thyroid receptor-interacting protein 6), also known as ZRP-1 (zyxin-related protein 1), is a member of the zyxin family that has been implicated in actin reorganization and cell motility. Trip6 has been proposed to transport signals from the cell surface to the nucleus.

Protocols

Product Specific Protocols
WB protocol for TRIP6 antibody 21163-1-APDownload protocol
IF protocol for TRIP6 antibody 21163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols