Validation Data Gallery
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Tested Applications
| Positive WB detected in | Hela cells |
| Positive IHC detected in | human ovary tumor tissue, human liver tissue, human colon cancer tissue, human colon tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
60205-1-Ig targets TRIP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TRIP6 fusion protein Ag15149 相同性解析による交差性が予測される生物種 |
| Full Name | thyroid hormone receptor interactor 6 |
| Calculated molecular weight | 476 aa, 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC004249 |
| Gene Symbol | TRIP6 |
| Gene ID (NCBI) | 7205 |
| RRID | AB_10973674 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15654 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRIP6 (thyroid receptor-interacting protein 6), also known as ZRP-1 (zyxin-related protein 1), is a member of the zyxin family that has been implicated in actin reorganization and cell motility. Trip6 has been proposed to transport signals from the cell surface to the nucleus.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIP6 antibody 60205-1-Ig | Download protocol |
| IHC protocol for TRIP6 antibody 60205-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex. |