Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17141-1-AP targets TRIT1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRIT1 fusion protein Ag10906 相同性解析による交差性が予測される生物種 |
| Full Name | tRNA isopentenyltransferase 1 |
| Calculated molecular weight | 467 aa, 53 kDa |
| Observed molecular weight | 53-60 kDa |
| GenBank accession number | BC107569 |
| Gene Symbol | TRIT1 |
| Gene ID (NCBI) | 54802 |
| RRID | AB_3669261 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3H1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IPTases (TRIT1) belong to a group of enzymes that modify both cytoplasmic and mitochondrial tRNAs of eukaryotic cells. Pathogenic mutations to human IPTase (TRIT1) cause mitochondrial insufficiency and neurodevelopmental disease, principally due to decreased modification of the mt-tRNA substrates.(PMID: 32324744, PMID: 36049610)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIT1 antibody 17141-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |