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TRMT1 Polyclonal antibody

TRMT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14970-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

tRNA(guanine-26,N(2)-N(2)) methyltransferase, tRNA 2,2-dimethylguanosine-26 methyltransferase, tRNA (guanine(26)-N(2))-dimethyltransferase, TRMT 1, TRM1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14970-1-AP targets TRMT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRMT1 fusion protein Ag6837 相同性解析による交差性が予測される生物種
Full Name TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
Calculated molecular weight 81 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC002492
Gene Symbol TRMT1
Gene ID (NCBI) 55621
RRIDAB_2209504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXH9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRMT1 is an RNA methyltransferase responsible for the formation of N2,N2-dimethylguanosine (m2,2G) at position 26 in both cytosolic and mitochondrial tRNAs.TRMT1-catalyzed tRNA modifications are important for maintenance of global protein translation levels including proteins involved in cellular growth, development, and stress response. Human neural stem cells with TRMT1 knockdowns were found to have hypersensitivity to redox stress, implicating TRMT1 and the m2,2G26 modification in the regulation of redox homeostasis.

Protocols

Product Specific Protocols
WB protocol for TRMT1 antibody 14970-1-APDownload protocol
IHC protocol for TRMT1 antibody 14970-1-APDownload protocol
IF protocol for TRMT1 antibody 14970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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