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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRMT1 Polyclonal antibody

TRMT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14970-1-AP

Synonyms

tRNA(guanine-26,N(2)-N(2)) methyltransferase, tRNA 2,2-dimethylguanosine-26 methyltransferase, tRNA (guanine(26)-N(2))-dimethyltransferase, TRMT 1, TRM1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TRMT1 Polyclonal antibody (14970-1-AP)

    WB analysis of HeLa using 14970-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14970-1-AP (TRMT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRMT1 Polyclonal antibody (14970-1-AP)

    IHC staining of human colon cancer using 14970-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14970-1-AP (TRMT1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRMT1 Polyclonal antibody (14970-1-AP)

    IHC staining of human colon cancer using 14970-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14970-1-AP (TRMT1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRMT1 Polyclonal antibody (14970-1-AP)

    IF Staining of HeLa using 14970-1-AP

    Immunofluorescent analysis of HeLa cells using 14970-1-AP (TRMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14970-1-AP targets TRMT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRMT1 fusion protein Ag6837 相同性解析による交差性が予測される生物種
Full Name TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
Calculated molecular weight 81 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC002492
Gene symbol TRMT1
Gene ID (NCBI) 55621
RRIDAB_2209504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRMT1 is an RNA methyltransferase responsible for the formation of N2,N2-dimethylguanosine (m2,2G) at position 26 in both cytosolic and mitochondrial tRNAs.TRMT1-catalyzed tRNA modifications are important for maintenance of global protein translation levels including proteins involved in cellular growth, development, and stress response. Human neural stem cells with TRMT1 knockdowns were found to have hypersensitivity to redox stress, implicating TRMT1 and the m2,2G26 modification in the regulation of redox homeostasis.

Protocols

Product Specific Protocols
WB protocol for TRMT1 antibody 14970-1-APDownload protocol
IHC protocol for TRMT1 antibody 14970-1-APDownload protocol
IF protocol for TRMT1 antibody 14970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols