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  • Featured Product
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TRMT11 Polyclonal antibody

TRMT11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17555-1-AP

Synonyms

tRNA guanosine-2'-O-methyltransferase TRM11 homolog, tRNA (guanine(10)-N2)-methyltransferase homolog, TRM11, MDS024, EC:2.1.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRMT11 Polyclonal antibody (17555-1-AP)

    WB analysis using 17555-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TRMT11 Polyclonal antibody (17555-1-AP)

    WB analysis of HEK-293 using 17555-1-AP

    WB result of TRMT11 antibody (17555-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT11 transfected HEK-293 cells.

  • Western Blot (WB) analysis of LNCaP cells using TRMT11 Polyclonal antibody (17555-1-AP)

    WB analysis of LNCaP using 17555-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TRMT11 Polyclonal antibody (17555-1-AP)

    WB analysis of HEK-293 using 17555-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TRMT11 Polyclonal antibody (17555-1-AP)

    WB analysis of K-562 using 17555-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using TRMT11 Polyclonal antibody (17555-1-AP)

    WB analysis of mouse testis using 17555-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17555-1-AP (TRMT11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRMT11 Polyclonal antibody (17555-1-AP)

    IHC staining of human liver using 17555-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17555-1-AP (TRMT11 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TRMT11 Polyclonal antibody (17555-1-AP)

    IHC staining of human liver using 17555-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17555-1-AP (TRMT11 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TRMT11 Polyclonal antibody (17555-1-AP)

    IF Staining of HepG2 using 17555-1-AP

    Immunofluorescent analysis of HepG2 cells, using IFT52 antibody 17555-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells, LNCaP cells, K-562 cells, mouse testis tissue, HepG2 cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17555-1-AP targets TRMT11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRMT11 fusion protein Ag11717 相同性解析による交差性が予測される生物種
Full Name tRNA methyltransferase 11 homolog (S. cerevisiae)
Calculated molecular weight 463 aa, 53 kDa
Observed molecular weight 48-53 kDa
GenBank accession numberBC056878
Gene symbol TRMT11
Gene ID (NCBI) 60487
RRIDAB_2209511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRMT11 antibody 17555-1-APDownload protocol
IHC protocol for TRMT11 antibody 17555-1-APDownload protocol
IF protocol for TRMT11 antibody 17555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Microbiol Immunol

BUD23-TRMT112 interacts with the L protein of Borna disease virus and mediates the chromosomal tethering of viral ribonucleoproteins.

Authors - Bea Clarise B Garcia
View Article