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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRMT112 Polyclonal antibody

TRMT112 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26472-1-AP

Synonyms

HSPC152, HSPC170, hypothetical protein HSPC152, TRM112 like protein, TRMT112

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRMT112 Polyclonal antibody (26472-1-AP)

    WB analysis using 26472-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26472-1-AP (TRMT112 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TRMT112 Polyclonal antibody (26472-1-AP)

    IHC staining of human ovary tumor using 26472-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26472-1-AP (TRMT112 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TRMT112 Polyclonal antibody (26472-1-AP)

    IHC staining of human ovary tumor using 26472-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26472-1-AP (TRMT112 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, PC-3 cells, SH-SY5Y cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26472-1-AP targets TRMT112 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRMT112 fusion protein Ag24126 相同性解析による交差性が予測される生物種
Full Name hypothetical protein HSPC152
Observed molecular weight14 kDa
GenBank accession numberBC017172
Gene symbol TRMT112
Gene ID (NCBI) 51504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRMT112 is a small evolutionarily conserved protein that acts as a co-factor and activator for different MTases involved in rRNA, tRNA and protein methylation. The TRMT112 interaction with 18S rRNA MTases WBSCR22 (BUD23, MERM1) and METTL5 is required for their metabolic stability and enzymatic activity in cells. (PMID: 34948388, PMID: 34948388)

Protocols

Product Specific Protocols
WB protocol for TRMT112 antibody 26472-1-APDownload protocol
IHC protocol for TRMT112 antibody 26472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

SPC25 overexpression promotes tumor proliferation and is prognostic of poor survival in hepatocellular carcinoma.

Authors - Baozhu Zhang
View Article