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  • Featured Product
  • KD/KO Validated

HTF9C Polyclonal antibody

HTF9C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16199-1-AP

Synonyms

HTF9C, TRMT2A

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using HTF9C Polyclonal antibody (16199-1-AP)

    WB analysis of MCF-7 using 16199-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16199-1-AP (HTF9C antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using HTF9C Polyclonal antibody (16199-1-AP)

    WB analysis of mouse liver using 16199-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16199-1-AP (HTF9C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HTF9C Polyclonal antibody (16199-1-AP)

    WB analysis of HepG2 using 16199-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16199-1-AP (HTF9C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HTF9C Polyclonal antibody (16199-1-AP)

    IHC staining of human kidney using 16199-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HTF9C Polyclonal antibody (16199-1-AP)

    IHC staining of human kidney using 16199-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HTF9C Polyclonal antibody (16199-1-AP)

    IHC staining of human breast cancer using 16199-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HTF9C Polyclonal antibody (16199-1-AP)

    IHC staining of human breast cancer using 16199-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HTF9C Polyclonal antibody (16199-1-AP)

    IHC staining of human kidney using 16199-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HTF9C Polyclonal antibody (16199-1-AP)

    IHC staining of human kidney using 16199-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16199-1-AP (HTF9C antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inMCF-7 cells, HepG2 cells, mouse liver tissue
Positive IHC detected inhuman kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16199-1-AP targets HTF9C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HTF9C fusion protein Ag9112 相同性解析による交差性が予測される生物種
Full Name TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
Calculated molecular weight 625 aa, 69 kDa
Observed molecular weight 42-44 kDa, 68-75 kDa
GenBank accession numberBC013352
Gene symbol TRMT2A
Gene ID (NCBI) 27037
RRIDAB_2209623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

tRNA methyltransferase 2 homolog A (TRMT2A), also known as HTF9C is a novel cell cycle regulated protein, as associated with aggressive clinical course. HTF9C harbors a putative RNA recognition motif (RRM) in the N-terminus and the conserved uracil-C5-methyltransferase domain of the TrmA family in the C-terminus. HTF9C antibody can detect two bands about 68 kDa and 40 kDa (PMID: 20307320).

Protocols

Product Specific Protocols
WB protocol for HTF9C antibody 16199-1-APDownload protocol
IHC protocol for HTF9C antibody 16199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.

Authors - Wei Dai
  • KD Validated
View Article