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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRMT2A Recombinant antibody (83463-8-RR)

    WB analysis using 83463-8-RR (same clone as 83463-8-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83463-8-RR (TRMT2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83463-8-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TRMT2A Recombinant antibody (83463-8-RR)

    IHC staining of mouse liver using 83463-8-RR (same clone as 83463-8-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83463-8-RR (TRMT2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83463-8-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using TRMT2A Recombinant antibody (83463-8-RR)

    FC experiment of U-251 using 83463-8-RR (same clone as 83463-8-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug TRMT2A Recombinant antibody (83463-8-RR, Clone:240444C2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83463-8-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83463-8-RR

    Affinity and Kinetic Characterization of 83463-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83463-8-RR against Human TRMT2A were performed. The affinity constant is 1.86 nM.

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TRMT2A Recombinant antibody, PBS Only

TRMT2A Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 83463-8-PBS
Clone No.240444C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

tRNA (uracil 5 ) methyltransferase homolog A, mRNA, HTF9C, EC:2.1.1.35, EC:2.1.1.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83463-8-PBS targets TRMT2A in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRMT2A fusion protein Ag9112 相同性解析による交差性が予測される生物種
Full Name TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae)
Calculated molecular weight 625 aa, 69 kDa
Observed molecular weight 42-44 kDa, 68-75 kDa
GenBank accession numberBC013352
Gene Symbol TRMT2A
Gene ID (NCBI) 27037
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IZ69
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

tRNA methyltransferase 2 homolog A (TRMT2A), also known as HTF9C is a novel cell cycle regulated protein, as associated with aggressive clinical course. HTF9C harbors a putative RNA recognition motif (RRM) in the N-terminus and the conserved uracil-C5-methyltransferase domain of the TrmA family in the C-terminus. HTF9C antibody can detect two bands about 68 kDa and 40 kDa (PMID: 20307320).