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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TROVE2 Polyclonal antibody

TROVE2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12718-1-AP

Synonyms

SS A, RoRNP, Ro60 autoantigen, RO60, RNA-binding protein RO60

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using TROVE2 Polyclonal antibody (12718-1-AP)

    WB analysis of MCF-7 using 12718-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TROVE2 Polyclonal antibody (12718-1-AP)

    WB analysis of HeLa using 12718-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TROVE2 Polyclonal antibody (12718-1-AP)

    WB analysis of PC-3 using 12718-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using TROVE2 Polyclonal antibody (12718-1-AP)

    WB analysis of rat spleen using 12718-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using TROVE2 Polyclonal antibody (12718-1-AP)

    WB analysis of mouse thymus using 12718-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12718-1-AP (TROVE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using TROVE2 Polyclonal antibody (12718-1-AP)

    IF Staining of PC-3 using 12718-1-AP

    Immunofluorescent analysis of PC-3 cells using 12718-1-AP (TROVE2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, mouse thymus tissue, PC-3 cells, rat spleen tissue
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12718-1-AP targets TROVE2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TROVE2 fusion protein Ag3412 相同性解析による交差性が予測される生物種
Full Name TROVE domain family, member 2
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC036658
Gene symbol TROVE2
Gene ID (NCBI) 6738
RRIDAB_2209756
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TROVE2, also named as RO60 or RoRNP, is a 538 amino acid protein, which is localized in cytoplasmic mRNP granules containing untranslated mRNAs. TROVE2 as a RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs. TROVE2 may stabilize some of these RNAs and protect them from degradation (PubMed:18056422). TROVE2 binds to endogenous Alu retroelements which are induced by type I IFN and stimulate porinflammaotry cytokine secretion.

Protocols

Product Specific Protocols
WB protocol for TROVE2 antibody 12718-1-APDownload protocol
IF protocol for TROVE2 antibody 12718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression.

Authors - Chuang Li
View Article