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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRPA1 Polyclonal antibody, PBS Only

TRPA1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19124-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TRPA1 Polyclonal antibody (19124-1-AP)

    WB analysis using 19124-1-AP (same clone as 19124-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19124-1-AP (TRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using TRPA1 Polyclonal antibody (19124-1-AP)

    IHC staining of mouse cerebellum using 19124-1-AP (same clone as 19124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using TRPA1 Polyclonal antibody (19124-1-AP)

    IHC staining of mouse cerebellum using 19124-1-AP (same clone as 19124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat dorsal root ganglion tissue using TRPA1 Polyclonal antibody (19124-1-AP)

    IHC staining of rat dorsal root ganglion using 19124-1-AP (same clone as 19124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRPA1 Polyclonal antibody (19124-1-AP)

    IHC staining of mouse brain using 19124-1-AP (same clone as 19124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRPA1 Polyclonal antibody (19124-1-AP)

    IHC staining of mouse brain using 19124-1-AP (same clone as 19124-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19124-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19124-1-PBS targets TRPA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily A, member 1
Calculated molecular weight 140 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberNM_007332
Gene symbol TRPA1
Gene ID (NCBI) 8989
RRIDAB_10642143
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TRPA1, also named as ANKTM1, belongs to the transient receptor family. TRPA1 is a receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. TRPA1 has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. It acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. It may be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds.