• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRPC1 Polyclonal antibody

TRPC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24727-1-AP

Synonyms

HTRP 1, Transient receptor protein 1, TRP 1, TRP1, TRPC1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TRPC1 Polyclonal antibody (24727-1-AP)

    WB analysis using 24727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24727-1-AP (TRPC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TRPC1 Polyclonal antibody (24727-1-AP)

    WB analysis using 24727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24727-1-AP (TRPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using TRPC1 Polyclonal antibody (24727-1-AP)

    IHC staining of rat brain using 24727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24727-1-AP (TRPC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using TRPC1 Polyclonal antibody (24727-1-AP)

    IHC staining of rat brain using 24727-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24727-1-AP (TRPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

24727-1-AP targets TRPC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Reactivity human, mouse , rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPC1 fusion protein Ag16488 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily C, member 1
Calculated molecular weight 793 aa, 91 kDa
Observed molecular weight120 kDa
GenBank accession numberBC113953
Gene symbol TRPC1
Gene ID (NCBI) 7220
RRIDAB_3085750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPC1, also named as TRP1, belongs to the transient receptor family and STrpC subfamily. TRPC1 is thought to form a receptor-activated non-selective calcium permeant cation channel. It has been reported that the mGluR1 evoked slow EPSC is mediated by the TRPC1 cation channel. It is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors.

Protocols

Product Specific Protocols
WB protocol for TRPC1 antibody 24727-1-APDownload protocol
IHC protocol for TRPC1 antibody 24727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Upregulation of TRPC1 protects against high glucose-induced HUVECs dysfunction by inhibiting oxidative stress

Authors - Mengting Qu
View Article