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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRPC4AP Polyclonal antibody

TRPC4AP Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 29310-1-AP

Synonyms

Trpc4-associated protein, Trp4-associated protein, TNF-receptor ubiquitous scaffolding/signaling protein, Short transient receptor potential channel 4-associated protein, PPP1R158

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRPC4AP Polyclonal antibody (29310-1-AP)

    WB analysis using 29310-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29310-1-AP (TRPC4AP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TRPC4AP Polyclonal antibody (29310-1-AP)

    IP experiment of HepG2 using 29310-1-AP

    IP result of anti-TRPC4AP (IP:29310-1-AP, 4ug; Detection:29310-1-AP 1:800) with HepG2 cells lysate 2040 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TRPC4AP Polyclonal antibody (29310-1-AP)

    IHC staining of human ovary cancer using 29310-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29310-1-AP (TRPC4AP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRPC4AP Polyclonal antibody (29310-1-AP)

    IHC staining of human colon cancer using 29310-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29310-1-AP (TRPC4AP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29310-1-AP targets TRPC4AP in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPC4AP fusion protein Ag29860 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily C, member 4 associated protein
Calculated molecular weight 797 aa, 91 kDa
Observed molecular weight85-90 kDa
GenBank accession numberBC013144
Gene symbol TRPC4AP
Gene ID (NCBI) 26133
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.

Protocols

Product Specific Protocols
WB protocol for TRPC4AP antibody 29310-1-APDownload protocol
IHC protocol for TRPC4AP antibody 29310-1-APDownload protocol
IP protocol for TRPC4AP antibody 29310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols