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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRPC4AP Monoclonal antibody

TRPC4AP Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C10B1

Cat no : 66138-1-Ig

Synonyms

C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using TRPC4AP Monoclonal antibody (66138-1-Ig)

    WB analysis of human brain using 66138-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66138-1-Ig (TRPC4AP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using TRPC4AP Monoclonal antibody (66138-1-Ig)

    WB analysis of human heart using 66138-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66138-1-Ig (TRPC4AP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRPC4AP Monoclonal antibody (66138-1-Ig)

    IHC staining of human liver using 66138-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66138-1-Ig(TRPC4AP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRPC4AP Monoclonal antibody (66138-1-Ig)

    IHC staining of human breast cancer using 66138-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66138-1-Ig (TRPC4AP Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRPC4AP Monoclonal antibody (66138-1-Ig)

    IHC staining of human breast cancer using 66138-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66138-1-Ig (TRPC4AP Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRPC4AP Monoclonal antibody (66138-1-Ig)

    IHC staining of human liver using 66138-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66138-1-Ig(TRPC4AP antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inhuman brain tissue, human heart tissue
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66138-1-Ig is TRPC4AP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen TRPC4AP fusion protein Ag18772 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily C, member 4 associated protein
Calculated molecular weight 797 aa, 91 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC013144
Gene symbol TRPC4AP
Gene ID (NCBI) 26133
RRIDAB_2881537
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.

Protocols

Product Specific Protocols
WB protocol for TRPC4AP antibody 66138-1-IgDownload protocol
IHC protocol for TRPC4AP antibody 66138-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols