TRPC5 Polyclonal antibody
TRPC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 25890-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, MCF-7 cells |
| Positive IHC detected in | human brain tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
Product Information
25890-1-AP targets TRPC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRPC5 fusion protein Ag23171 相同性解析による交差性が予測される生物種 |
| Full Name | transient receptor potential cation channel, subfamily C, member 5 |
| Calculated molecular weight | 111 kDa |
| Observed molecular weight | 105 kDa |
| GenBank accession number | BC137271 |
| Gene symbol | TRPC5 |
| Gene ID (NCBI) | 7224 |
| RRID | AB_2880285 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Short transient receptor potential channel 5(TRPC5), which belongs to TRPC5 sub-subfamily of transient receptor (TC 1.A.4) family, thought to form a receptor-activated non-selective calcium permeant cation channel, and probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC5 has also been shown to be calcium-selective and may also be activated by intracellular calcium store depletion.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRPC5 antibody 25890-1-AP | Download protocol |
| IHC protocol for TRPC5 antibody 25890-1-AP | Download protocol |
| IF protocol for TRPC5 antibody 25890-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin Endothelial cell Orai1 is essential for endothelium-dependent contraction of mouse carotid arteries in normotensive and hypertensive mice | ||
Cancer Lett TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway. | ||
J Neuroinflammation Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice. | ||
J Mol Cell Cardiol TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes. | ||
Biochem Pharmacol Endothelial cell transient receptor potential channel C5 (TRPC5) is essential for endothelium-dependent contraction in mouse carotid arteries. |
