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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRPM2 Polyclonal antibody

TRPM2 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 21842-1-AP

Synonyms

LTrpC 2, KNP3, KNP 3, Estrogen-responsive element-associated gene 1 protein, EREG1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRPM2 Polyclonal antibody (21842-1-AP)

    IHC staining of mouse brain using 21842-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21842-1-AP (TRPM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRPM2 Polyclonal antibody (21842-1-AP)

    IHC staining of mouse brain using 21842-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21842-1-AP (TRPM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TRPM2 Polyclonal antibody (21842-1-AP)

    IF Staining of mouse brain using 21842-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRPM2 antibody (21842-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TRPM2 Polyclonal antibody (21842-1-AP)

    IF Staining of mouse brain using 21842-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRPM2 antibody (21842-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21842-1-AP targets TRPM2 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPM2 fusion protein Ag16522 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily M, member 2
Calculated molecular weight 1553 aa, 177 kDa
GenBank accession numberBC112342
Gene symbol TRPM2
Gene ID (NCBI) 7226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for TRPM2 antibody 21842-1-APDownload protocol
IF protocol for TRPM2 antibody 21842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols