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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRPM3 Polyclonal antibody (12724-1-AP)

    WB analysis using 12724-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12724-1-AP (TRPM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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TRPM3 Polyclonal antibody

TRPM3 Polyclonal Antibody for WB, ELISA
Cat No. 12724-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

GON 2, LTRPC3, MLSN2, TRPM3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12724-1-AP targets TRPM3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPM3 fusion protein Ag3421 相同性解析による交差性が予測される生物種
Full Name transient receptor potential cation channel, subfamily M, member 3
Calculated molecular weight 1732 aa, 197 kDa
Observed molecular weight160-210 kDa
GenBank accession numberBC022454
Gene Symbol TRPM3
Gene ID (NCBI) 80036
RRIDAB_2935431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCF6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transient receptor potential melastatin-3 (TRPM3) channel belongs to the family of transient receptor potential (TRP) cation channels that are expressed in a variety of tissues and cell types, including kidney, brain, dorsal root ganglia, cardiomyocytes and pancreatic beta-cells. TRPM3 channels can be activated by the neurosteroid pregnenolone sulfate, and they are regulated by phosphoinositides, and perhaps by calmodulin. Stimulation of TRPM3 channels is connected with heat sensation by somatosensory neurons, insulin secretion by pancreatic beta-cells, regulation of neurotransmitter release, iris constriction, and tumor promotion. The calculated MW of TRPM3 is 197 kDa, 12724-1-AP can detect a band around 160 kDa, which likely corresponds to different splice variant. (PMID: 28720517,21198543)

Protocols

Product Specific Protocols
WB protocol for TRPM3 antibody 12724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols