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KD/KO Validated

TRPS1 Polyclonal antibody

Name: TRPS1 Polyclonal antibody
Brand: Proteintech
SKU: 21938-1-AP

TRPS1 Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21938-1-AP

Synonyms

GC79, LGCR, TRPS1, Zinc finger protein GC79

Validation Data Gallery

Filter:

AllWBIPIF-P

WB analysis using 21938-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21938-1-AP
WB result of TRPS1 antibody (21938-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRPS1 transfected HEK-293 cells.
WB analysis of HeLa using 21938-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21938-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 21938-1-AP
IP result of anti-TRPS1 (IP:21938-1-AP, 4ug; Detection:21938-1-AP 1:1000) with mouse lung tissue lysate 3600ug.
IF Staining of mouse skin using 21938-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse skin using 21938-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 21938-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, mouse kidney tissue, mouse lung tissue, mouse testis tissue
Positive IP detected in	mouse lung tissue
Positive IF-P detected in	mouse skin tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IF	See 2 publications below

Product Information

21938-1-AP targets TRPS1 in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRPS1 fusion protein Ag16583 相同性解析による交差性が予測される生物種
Full Name	trichorhinophalangeal syndrome I
Calculated molecular weight	1294 aa, 143 kDa
Observed molecular weight	150 kDa
GenBank accession number	BC125020
Gene symbol	TRPS1
Gene ID (NCBI)	7227
RRID	AB_11232233
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains

Protocols

Product Specific Protocols
WB protocol for TRPS1 antibody 21938-1-AP	Download protocol
IF protocol for TRPS1 antibody 21938-1-AP	Download protocol
IP protocol for TRPS1 antibody 21938-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Biochim Biophys Acta Mol Basis Dis UVB-induced TRPS1 regulates MITF transcription activity to promote skin pigmentation Authors - Yushan Zhang View Article
mouse	IF	Acta Biomater Shape-Fixing Hydrogel Promotes Scarless Healing of Wounds Under Tension Authors - Danlan Fu View Article
human	IF	Clin Transl Oncol Endoplasmic reticulum stress regulates apoptosis and chemotherapeutic via enhancing TNFRSF10B recycling to the cell membrane in triple-negative breast cancer Authors - Dapeng Zhao View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRPS1 Polyclonal antibody

TRPS1 Polyclonal Antibody for WB, IF-P, IP, ELISA

Validation Data Gallery

WB analysis using 21938-1-AP

WB analysis of HEK-293 using 21938-1-AP

WB analysis of HeLa using 21938-1-AP

WB analysis of HEK-293 using 21938-1-AP

IP experiment of mouse lung using 21938-1-AP

IF Staining of mouse skin using 21938-1-AP

IF Staining of mouse skin using 21938-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

UVB-induced TRPS1 regulates MITF transcription activity to promote skin pigmentation

Shape-Fixing Hydrogel Promotes Scarless Healing of Wounds Under Tension

Endoplasmic reticulum stress regulates apoptosis and chemotherapeutic via enhancing TNFRSF10B recycling to the cell membrane in triple-negative breast cancer