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  • Featured Product
  • KD/KO Validated

TRPS1 Polyclonal antibody

TRPS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28753-1-AP

Synonyms

Tricho-rhino-phalangeal syndrome type I protein, LGCR, GC79

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRPS1 Polyclonal antibody (28753-1-AP)

    WB analysis using 28753-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28753-1-AP (TRPS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TRPS1 Polyclonal antibody (28753-1-AP)

    IHC staining of human ovary cancer using 28753-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28753-1-AP (TRPS1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TRPS1 Polyclonal antibody (28753-1-AP)

    IHC staining of human ovary cancer using 28753-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28753-1-AP (TRPS1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28753-1-AP targets TRPS1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPS1 fusion protein Ag30188 相同性解析による交差性が予測される生物種
Full Name trichorhinophalangeal syndrome I
Calculated molecular weight 142 kDa
Observed molecular weight142 kDa
GenBank accession numberNM_014112
Gene symbol TRPS1
Gene ID (NCBI) 7227
RRIDAB_2881209
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains

Protocols

Product Specific Protocols
WB protocol for TRPS1 antibody 28753-1-APDownload protocol
IHC protocol for TRPS1 antibody 28753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols