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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRPS1 Recombinant antibody

TRPS1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230051F10

Cat no : 83062-4-RR

Synonyms

Tricho-rhino-phalangeal syndrome type I protein, LGCR, GC79, 230051F10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRPS1 Recombinant antibody (83062-4-RR)

    WB analysis using 83062-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83062-4-RR (TRPS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRPS1 Recombinant antibody (83062-4-RR)

    IHC staining of human breast cancer using 83062-4-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 83062-4-RR (TRPS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of MCF-7 cells using TRPS1 Recombinant antibody (83062-4-RR)

    FC experiment of MCF-7 using 83062-4-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TRPS1 Recombinant antibody (83062-4-RR, Clone:230051F10)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83062-4-RR

    Affinity and Kinetic Characterization of 83062-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83062-4-RR against Human TRPS1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83062-4-RR targets TRPS1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRPS1 fusion protein Ag33959 相同性解析による交差性が予測される生物種
Full Name trichorhinophalangeal syndrome I
Calculated molecular weight 1294 aa, 143 kDa
Observed molecular weight150 kDa
GenBank accession numberBC125020
Gene symbol TRPS1
Gene ID (NCBI) 7227
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains.

Protocols

Product Specific Protocols
WB protocol for TRPS1 antibody 83062-4-RRDownload protocol
IHC protocol for TRPS1 antibody 83062-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols