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TRUB2 Polyclonal antibody

TRUB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19891-1-AP

Synonyms

CLONE24922, RP11 339B21.1, TRUB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRUB2 Polyclonal antibody (19891-1-AP)

    WB analysis using 19891-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TRUB2 Polyclonal antibody (19891-1-AP)

    WB analysis of HeLa using 19891-1-AP

    WB result of TRUB2 antibody (19891-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRUB2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRUB2 Polyclonal antibody (19891-1-AP)

    IHC staining of human colon cancer using 19891-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRUB2 Polyclonal antibody (19891-1-AP)

    IHC staining of human colon cancer using 19891-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

19891-1-AP targets TRUB2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRUB2 fusion protein Ag13726 相同性解析による交差性が予測される生物種
Full Name TruB pseudouridine (psi) synthase homolog 2 (E. coli)
Calculated molecular weight 331 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC001457
Gene symbol TRUB2
Gene ID (NCBI) 26995
RRIDAB_10640900
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRUB2 antibody 19891-1-APDownload protocol
IHC protocol for TRUB2 antibody 19891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

Authors - Hana Antonicka
View Article
humanWB

Nat Cell Biol

Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression

Authors - Nicholas J Kramer
View Article