Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
12352-1-AP targets GILZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GILZ fusion protein Ag3015 相同性解析による交差性が予測される生物種 |
| Full Name | TSC22 domain family, member 3 |
| Calculated molecular weight | 134 aa, 15 kDa |
| Observed molecular weight | 17 kDa |
| GenBank accession number | BC018148 |
| Gene Symbol | GILZ |
| Gene ID (NCBI) | 1831 |
| RRID | AB_2272196 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99576 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GILZ, also known as TSC22D3 and DSIPI, is a glucocorticoid responsive protein and is thought to mediate some of the anti-inflammatory effects of glucocorticoid receptors (GRs). GILZ is mainly localized in the cytoplasm. It is an anti-inflammatory molecule that could mediate the immunomodulatory activities of GCs and also plays a role in cell differentiation in various cells and tissues (PMID: 35011684).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GILZ antibody 12352-1-AP | Download protocol |
| IHC protocol for GILZ antibody 12352-1-AP | Download protocol |
| IF protocol for GILZ antibody 12352-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci Glucocorticoid-Induced Leucine Zipper Protects the Retina From Light-Induced Retinal Degeneration by Inducing Bcl-xL in Rats. | ||
Invest Ophthalmol Vis Sci Glucocorticoid-Induced Leucine Zipper Suppresses ICAM-1 and MCP-1 Expression by Dephosphorylation of NF-κB p65 in Retinal Endothelial Cells. | ||
Exp Eye Res Glucocorticoid-induced leucine zipper overexpression inhibits lipopolysaccharide-induced retinal inflammation in rats. |