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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TSFM Polyclonal antibody (11701-1-AP)

    WB analysis of HeLa using 11701-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TSFM Polyclonal antibody (11701-1-AP)

    WB analysis of PC-3 using 11701-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TSFM Polyclonal antibody (11701-1-AP)

    WB analysis of HEK-293 using 11701-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TSFM Polyclonal antibody (11701-1-AP)

    IHC staining of human colon cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TSFM Polyclonal antibody (11701-1-AP)

    IHC staining of human colon cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TSFM Polyclonal antibody (11701-1-AP)

    IHC staining of human kidney using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TSFM Polyclonal antibody (11701-1-AP)

    IHC staining of human kidney using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TSFM Polyclonal antibody (11701-1-AP)

    IHC staining of human stomach cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TSFM Polyclonal antibody (11701-1-AP)

    IHC staining of human stomach cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TSFM Polyclonal antibody (11701-1-AP)

    IF Staining of HeLa using 11701-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11701-1-AP (TSFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

TSFM Polyclonal antibody

TSFM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11701-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EF-TsMt, EF-Ts, EF Tsmt, EF TS, COXPD3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11701-1-AP targets TSFM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSFM fusion protein Ag2308 相同性解析による交差性が予測される生物種
Full Name Ts translation elongation factor, mitochondrial
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC022862
Gene Symbol TSFM
Gene ID (NCBI) 10102
RRIDAB_2207953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43897
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TSFM antibody 11701-1-APDownload protocol
IHC protocol for TSFM antibody 11701-1-APDownload protocol
IF protocol for TSFM antibody 11701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Biol Rep

Short-term regulation of TSFM level does not alter amyloidogenesis and mitochondrial function in type-specific cells

Authors - Xiao-Yun Li
  • KD Validated
View Article