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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSG-6 Recombinant antibody (83163-4-RR)

    WB analysis using 83163-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83163-4-RR (TNFAIP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using TSG-6 Recombinant antibody (83163-4-RR)

    FC experiment of A549 using 83163-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug TSG-6 Recombinant antibody (83163-4-RR, Clone:230438D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83163-4-RR

    Affinity and Kinetic Characterization of 83163-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83163-4-RR against Human TSG-6 were performed. The affinity constant is 1.93 nM.

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TSG-6 Recombinant antibody

TSG-6 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83163-4-RR
Clone No.230438D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

TNFAIP6, TNF alpha-induced protein 6, TNF alpha induced protein 6, Hyaluronate-binding protein, Hyaluronate binding protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, NIH/3T3 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83163-4-RR targets TSG-6 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TSG-6 fusion protein Ag3885 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor, alpha-induced protein 6
Calculated molecular weight 277 aa, 31 kDa
Observed molecular weight39-45 kDa
GenBank accession numberBC030205
Gene Symbol TNFAIP6
Gene ID (NCBI) 7130
RRIDAB_3670858
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP98066
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TSG-6 antibody 83163-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols