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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSG101 Monoclonal antibody (67381-1-Ig)

    WB analysis using 67381-1-Ig (same clone as 67381-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TSG101 Monoclonal antibody (67381-1-Ig)

    WB analysis using 67381-1-Ig (same clone as 67381-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human urine exosomes tissue using TSG101 Monoclonal antibody (67381-1-Ig)

    WB analysis of human urine exosomes using 67381-1-Ig (same clone as 67381-1-PBS)

    human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TSG101 Monoclonal antibody (67381-1-Ig)

    IHC staining of human lung cancer using 67381-1-Ig (same clone as 67381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TSG101 Monoclonal antibody (67381-1-Ig)

    IHC staining of human lung cancer using 67381-1-Ig (same clone as 67381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TSG101 Monoclonal antibody (67381-1-Ig)

    IHC staining of human colon cancer using 67381-1-Ig (same clone as 67381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TSG101 Monoclonal antibody (67381-1-Ig)

    IHC staining of human colon cancer using 67381-1-Ig (same clone as 67381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using TSG101 Monoclonal antibody (67381-1-Ig)

    IF Staining of human colon cancer using 67381-1-Ig (same clone as 67381-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TSG101 antibody (67381-1-Ig, Clone: 2B7G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TSG101 Monoclonal antibody (67381-1-Ig)

    IF Staining of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67381-1-Ig (TSG101 antibody), at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

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TSG101 Monoclonal antibody, PBS Only

TSG101 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67381-1-PBS
Clone No.2B7G8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67381-1-PBS targets TSG101 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TSG101 fusion protein Ag28569 相同性解析による交差性が予測される生物種
Full Name tumor susceptibility gene 101
Calculated molecular weight 44 kDa
Observed molecular weight 43-46 kDa
GenBank accession numberBC002487
Gene Symbol TSG101
Gene ID (NCBI) 7251
RRIDAB_2882628
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99816
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.