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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TSG101 Recombinant antibody

TSG101 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241657A1

Cat no : 84334-4-RR

Synonyms

VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSG101 Recombinant antibody (84334-4-RR)

    WB analysis using 84334-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84334-4-RR (TSG101 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TSG101 Recombinant antibody (84334-4-RR)

    IHC staining of human colon cancer using 84334-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84334-4-RR (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84334-4-RR

    Affinity and Kinetic Characterization of 84334-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84334-4-RR against Human TSG101 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, JurKat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84334-4-RR is TSG101 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TSG101 fusion protein Ag28569 相同性解析による交差性が予測される生物種
Full Name tumor susceptibility gene 101
Calculated molecular weight 44 kDa
Observed molecular weight44 kDa
GenBank accession numberBC002487
Gene symbol TSG101
Gene ID (NCBI) 7251
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Protocols

Product Specific Protocols
WB protocol for TSG101 antibody 84334-4-RRDownload protocol
IHC protocol for TSG101 antibody 84334-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols