Validation Data Gallery

Filter: AllWBIHC

WB analysis of rat testis using 12593-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12593-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 12593-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 12593-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12593-1-AP (TSGA10 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 12593-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12593-1-AP (TSGA10 Antibody) at dilution of 1:200 (under 40x lens).

TSGA10 Polyclonal antibody

TSGA10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12593-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

CEP4L, testis specific, 10, TSGA10

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	rat testis tissue, mouse testis tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 2 publications below
IF	See 3 publications below

Product Information

12593-1-AP targets TSGA10 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TSGA10 fusion protein Ag3311 相同性解析による交差性が予測される生物種
Full Name	testis specific, 10
Calculated molecular weight	698 aa, 81 kDa
Observed molecular weight	81 kDa
GenBank accession number	BC028366
Gene Symbol	TSGA10
Gene ID (NCBI)	80705
RRID	AB_2240967
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BZW7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TSGA10 antibody 12593-1-AP	Download protocol
IHC protocol for TSGA10 antibody 12593-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IHC	Front Cell Dev Biol miR-129-5p Inhibits Bone Formation Through TCF4. Authors - Chong Yin View Article
human	WB	Front Cell Dev Biol Pathogenic Variants in ACTRT1 Cause Acephalic Spermatozoa Syndrome. Authors - Yanwei Sha View Article
human,mouse	WB，IHC	J Cell Mol Med MACF1 alleviates aging-related osteoporosis via HES1. Authors - Chong Yin View Article
human	WB,IF	Clin Genet TSGA10 is a novel candidate gene associated with acephalic spermatozoa. Authors - Yan-Wei Sha View Article
human	WB	J Assist Reprod Genet Pathogenesis of acephalic spermatozoa syndrome caused by splicing mutation and de novo deletion in TSGA10. Authors - Mingfei Xiang View Article
human	WB,IF	Basic Clin Androl A novel homozygous TSGA10 missense variant causes acephalic spermatozoa syndrome in a Pakistani family Authors - Khalid Khan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of rat testis using 12593-1-AP

WB analysis of mouse testis using 12593-1-AP

WB analysis of rat testis using 12593-1-AP

IHC staining of human testis using 12593-1-AP

IHC staining of human testis using 12593-1-AP

TSGA10 Polyclonal antibody

TSGA10 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

miR-129-5p Inhibits Bone Formation Through TCF4.

Pathogenic Variants in ACTRT1 Cause Acephalic Spermatozoa Syndrome.

MACF1 alleviates aging-related osteoporosis via HES1.

TSGA10 is a novel candidate gene associated with acephalic spermatozoa.

Pathogenesis of acephalic spermatozoa syndrome caused by splicing mutation and de novo deletion in TSGA10.

A novel homozygous TSGA10 missense variant causes acephalic spermatozoa syndrome in a Pakistani family