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Thrombospondin 1 Polyclonal antibody

Name: Thrombospondin 1 Polyclonal antibody
Brand: Proteintech
SKU: 18304-1-AP
Rating: 5.00 (1 reviews)

Thrombospondin 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 18304-1-AP

Synonyms

Thrombospondin-1, THBS1, TSP, THBS 1, THBS

Validation Data Gallery

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AllWBIHC

WB analysis using 18304-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18304-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 18304-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 18304-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human peripheral blood platelets using 18304-1-AP
Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 18304-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 18304-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	C6 cells, A431 cells, human peripheral blood platelets, HeLa cells, HUVEC cells, NIH/3T3 cells
Positive IHC detected in	human lung cancer tissue, human placenta tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 35 publications below
IHC	See 13 publications below
IF	See 10 publications below

Product Information

18304-1-AP targets Thrombospondin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	thrombospondin 1
Calculated molecular weight	129 kDa
Observed molecular weight	180 kDa
GenBank accession number	BC028145
Gene symbol	Thrombospondin 1
Gene ID (NCBI)	7057
RRID	AB_2201959
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.

Protocols

Product Specific Protocols
WB protocol for Thrombospondin 1 antibody 18304-1-AP	Download protocol
IHC protocol for Thrombospondin 1 antibody 18304-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation. Authors - Yansen Xiao View Article
human	WB	Nat Commun The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets. Authors - Henry Nording View Article
mouse	IF	Nat Commun Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction. Authors - Juan Tang View Article
human	IHC	Cancer Lett Gastric cancer derived exosomal THBS1 enhanced Vγ9Vδ2 T-cell function through activating RIG-I-like receptor signaling pathway in a N6-methyladenosine methylation dependent manner Authors - Juntao Li View Article
rat	IF,WB	Transl Res Ntsr1 contributes to pulmonary hypertension by enhancing endoplasmic reticulum stress via JAK2-STAT3-Thbs1 signaling Authors - Zhi-Xing Wei View Article
human	IHC	Oncotarget Novel circulating peptide biomarkers for esophageal squamous cell carcinoma revealed by a magnetic bead-based MALDI-TOFMS assay. Authors - Kun Jia View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Emma (Verified Customer) (03-15-2022)

A single band by WB in DU145 cells. Used at 1:1000 @ 4C overnight.

Applications: Western Blot
Primary Antibody Dilution: 1:1000
Cell Tissue Type: Prostate

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Thrombospondin 1 Polyclonal antibody

Thrombospondin 1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 18304-1-AP

WB analysis of HeLa using 18304-1-AP

WB analysis using 18304-1-AP

WB analysis of A431 using 18304-1-AP

WB analysis of human peripheral blood platelets using 18304-1-AP

IHC staining of human lung cancer using 18304-1-AP

IHC staining of human lung cancer using 18304-1-AP

IHC staining of human placenta using 18304-1-AP

IHC staining of human liver using 18304-1-AP

IHC staining of human liver using 18304-1-AP

IHC staining of human placenta using 18304-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.

The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets.

Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction.

Gastric cancer derived exosomal THBS1 enhanced Vγ9Vδ2 T-cell function through activating RIG-I-like receptor signaling pathway in a N6-methyladenosine methylation dependent manner

Ntsr1 contributes to pulmonary hypertension by enhancing endoplasmic reticulum stress via JAK2-STAT3-Thbs1 signaling

Novel circulating peptide biomarkers for esophageal squamous cell carcinoma revealed by a magnetic bead-based MALDI-TOFMS assay.

Reviews

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