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KD/KO Validated

TSP50 Polyclonal antibody

Name: TSP50 Polyclonal antibody
Brand: Proteintech
SKU: 12574-1-AP

TSP50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12574-1-AP

Synonyms

testes protease 50, Serine protease 50, PRSS50, EC:3.4.25.-, CT20

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of MCF-7 using 12574-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12574-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12574-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 12574-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12574-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12574-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12574-1-AP (TSP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 12574-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12574-1-AP (TSP50 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 12574-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 12574-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 12574-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 12574-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12574-1-AP (TSP50 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 12574-1-AP
Immunofluorescent analysis of MCF-7 cells using 12574-1-AP (TSP50 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 12574-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF7 cells, HEK-293 cells, human testis tissue, MCF-7 cells, mouse testis tissue
Positive IHC detected in	human ovary tumor tissue, human breast cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 3 publications below

Product Information

12574-1-AP targets TSP50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TSP50 fusion protein Ag3247 相同性解析による交差性が予測される生物種
Full Name	testes-specific protease 50
Calculated molecular weight	385 aa, 43 kDa
Observed molecular weight	50-55 kDa
GenBank accession number	BC033016
Gene symbol	TSP50
Gene ID (NCBI)	29122
RRID	AB_10646475
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TSP50 (Testis-specific protease-like protein 50) is also named as PRSS50, CT20 and belongs to the peptidase S1 family. It can enhance the expression of NFKB target genes involved in cell proliferation, and promotes cell proliferation, at least partially, through activation of the NFKB signalling pathway(PMID:21385156). There are two protein bands of 37 kDa and 43 kDa to be detected in human testes that the the difference is most likely caused by posttranslational proteolytic cleavage(PMID:11782390). The full length protein has a signal peptide with 39 amino acids and two glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for TSP50 antibody 12574-1-AP	Download protocol
IHC protocol for TSP50 antibody 12574-1-AP	Download protocol
IF protocol for TSP50 antibody 12574-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	BMC Cancer Testes-specific protease 50 (TSP50) promotes invasion and metastasis by inducing EMT in gastric cancer. Authors - Qing-Hua Cao KD Validated View Article
		Oncol Rep Lentivirus-mediated knockdown of TSP50 suppresses the growth of non-small cell lung cancer cells via G0/G1 phase arrest. Authors - Wen-Liang Qiao View Article
human	WB,IHC	Gastroenterol Res Pract Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer. Authors - Fang Liu View Article
human	IHC	Oncol Lett Testes-specific protease 50 as an independent risk factor for poor prognosis in patients with non-small cell lung cancer. Authors - Wen-Liang Qiao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TSP50 Polyclonal antibody

TSP50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of MCF-7 using 12574-1-AP

WB analysis of mouse testis using 12574-1-AP

WB analysis of HEK-293 using 12574-1-AP

WB analysis of human testis using 12574-1-AP

WB analysis of MCF-7 using 12574-1-AP

WB analysis of MCF-7 using 12574-1-AP

IHC staining of human ovary tumor using 12574-1-AP

IHC staining of human testis using 12574-1-AP

IHC staining of human testis using 12574-1-AP

IHC staining of human breast cancer using 12574-1-AP

IHC staining of human breast cancer using 12574-1-AP

IF Staining of MCF-7 using 12574-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Testes-specific protease 50 (TSP50) promotes invasion and metastasis by inducing EMT in gastric cancer.

Lentivirus-mediated knockdown of TSP50 suppresses the growth of non-small cell lung cancer cells via G0/G1 phase arrest.

Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer.

Testes-specific protease 50 as an independent risk factor for poor prognosis in patients with non-small cell lung cancer.