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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TSPAN12 Polyclonal antibody (12812-1-AP)

    WB analysis of HepG2 using 12812-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12812-1-AP (TSPAN12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TSPAN12 Polyclonal antibody (12812-1-AP)

    WB analysis of MCF-7 using 12812-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12812-1-AP (TSPAN12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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TSPAN12 Polyclonal antibody

TSPAN12 Polyclonal Antibody for WB, ELISA
Cat No. 12812-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

NET 2, NET2, Tetraspan NET 2, tetraspanin 12, TM4SF12, Tspan 12, TSPAN12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12812-1-AP targets TSPAN12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSPAN12 fusion protein Ag3514 相同性解析による交差性が予測される生物種
Full Name tetraspanin 12
Calculated molecular weight 305 aa, 35 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC031265
Gene Symbol TSPAN12
Gene ID (NCBI) 23554
RRIDAB_2208667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95859
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSPAN12 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN12 regulates retinal vascular development by promoting Norrin/beta-catenin signaling (PMID: 19837033 ). Mutations in TSPAN12 are a relatively frequent cause of familial exudative vitreoretinopathy (FEVR) (PMID: 20159111).

Protocols

Product Specific Protocols
WB protocol for TSPAN12 antibody 12812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols