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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TSPAN14 Polyclonal antibody

TSPAN14 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15314-1-AP

Synonyms

DC TM4F2, tetraspanin 14, TM4SF14, Tspan 14, TSPAN14

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using TSPAN14 Polyclonal antibody (15314-1-AP)

    WB analysis of A549 using 15314-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15314-1-AP (TSPAN14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using TSPAN14 Polyclonal antibody (15314-1-AP)

    WB analysis of A549 using 15314-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15314-1-AP (TSPAN14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using TSPAN14 Polyclonal antibody (15314-1-AP)

    WB analysis of SH-SY5Y using 15314-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15314-1-AP (TSPAN14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15314-1-AP targets TSPAN14 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSPAN14 fusion protein Ag7551 相同性解析による交差性が予測される生物種
Full Name tetraspanin 14
Calculated molecular weight 31 kDa
Observed molecular weight31 kDa
GenBank accession numberBC002920
Gene symbol TSPAN14
Gene ID (NCBI) 81619
RRIDAB_2918039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSPAN14 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN14 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

Protocols

Product Specific Protocols
WB protocol for TSPAN14 antibody 15314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles

Authors - Liqiao Hu
View Article