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Validation Data Gallery

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  • Western Blot (WB) analysis of human ovary cancer cells using TSPAN15 Polyclonal antibody (10460-1-AP)

    WB analysis of human ovary cancer using 10460-1-AP

    human ovary cancer were subjected to SDS PAGE followed by western blot with 10460-1-AP (TSPAN15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

TSPAN15 Polyclonal antibody

TSPAN15 Polyclonal Antibody for WB, ELISA
Cat No. 10460-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Synonyms

2700063A19Rik, NET 7, NET7, Tetraspan NET 7, tetraspanin 15, TM4SF15, Tspan 15, TSPAN15

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman ovary cancer

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10460-1-AP targets TSPAN15 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSPAN15 fusion protein Ag0710 相同性解析による交差性が予測される生物種
Full Name tetraspanin 15
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC003157
Gene Symbol TSPAN15
Gene ID (NCBI) 23555
RRIDAB_2303826
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95858
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSPAN15 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN15 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN14, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

Protocols

Product Specific Protocols
WB protocol for TSPAN15 antibody 10460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Liver Int

Tspan15-ADAM10 signalling enhances cancer stem cell-like properties and induces chemoresistance via Notch1 activation in ICC

Authors - Arihito Yoshizumi
View Article