TSPAN15 Polyclonal antibody
TSPAN15 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10460-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human ovary cancer |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
10460-1-AP targets TSPAN15 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TSPAN15 fusion protein Ag0710 相同性解析による交差性が予測される生物種 |
Full Name | tetraspanin 15 |
Calculated molecular weight | 33 kDa |
Observed molecular weight | 33 kDa |
GenBank accession number | BC003157 |
Gene symbol | TSPAN15 |
Gene ID (NCBI) | 23555 |
RRID | AB_2303826 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSPAN15 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN15 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN14, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).
Protocols
Product Specific Protocols | |
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WB protocol for TSPAN15 antibody 10460-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Liver Int Tspan15-ADAM10 signalling enhances cancer stem cell-like properties and induces chemoresistance via Notch1 activation in ICC |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Maria (Verified Customer) (03-15-2022) | Some unspecific bands in the upper part of the gel but not so much at the expected molecular weight. I used 1:500 solution loading 30ug of protein, but I think it might be possible to use 1:1000.
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FH Boyan (Verified Customer) (10-04-2021) | calculated size at 33 and 23 kd. This antibody recognized a very weak band at 23 kd.
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