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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using TSPAN31 Polyclonal antibody (21987-1-AP)

    WB analysis of HeLa using 21987-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TSPAN31 Polyclonal antibody (21987-1-AP)

    WB analysis of HepG2 using 21987-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TSPAN31 Polyclonal antibody (21987-1-AP)

    WB analysis of PC-3 using 21987-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using TSPAN31 Polyclonal antibody (21987-1-AP)

    WB analysis of mouse skeletal muscle using 21987-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TSPAN31 Polyclonal antibody (21987-1-AP)

    IHC staining of human skeletal muscle using 21987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TSPAN31 Polyclonal antibody (21987-1-AP)

    IHC staining of human skeletal muscle using 21987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TSPAN31 Polyclonal antibody (21987-1-AP)

    IHC staining of human heart using 21987-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TSPAN31 Polyclonal antibody (21987-1-AP)

    IHC staining of human heart using 21987-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 40x lens).

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TSPAN31 Polyclonal antibody

TSPAN31 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21987-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Sarcoma amplified sequence, SAS, tetraspanin 31, Tspan 31, TSPAN31

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 450 μg/ml
20 μL, 450 μg/ml
150 μL, 450 μg/ml

¥67,000/ 150 μL

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse skeletal muscle tissue, PC-3 cells
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

21987-1-AP targets TSPAN31 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSPAN31 fusion protein Ag17138 相同性解析による交差性が予測される生物種
Full Name tetraspanin 31
Calculated molecular weight 210 aa, 23 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC010377
Gene Symbol TSPAN31
Gene ID (NCBI) 6302
RRIDAB_2878962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12999
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tetraspanin-31 (TSPAN31) belongs to the tetraspanin (TM4SF) family, also known as the transmembrane 4 (TM4) superfamily, which are a family of transmembrane proteins with 33 mammalian members. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. Tetraspanin-31 is thought to be involved in growth-related cellular processes. The experimentally determined molecular mass of 30 kDa is larger than the calculated molecular mass of 23 kDa, which could be a result of post-translational modifications.

Protocols

Product Specific Protocols
WB protocol for TSPAN31 antibody 21987-1-APDownload protocol
IHC protocol for TSPAN31 antibody 21987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Sci

Overexpression of Tetraspanin31 contributes to malignant potential and poor outcomes in gastric cancer

Authors - Yusuke Takashima
View Article