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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TSPAN6 Polyclonal antibody

TSPAN6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12293-1-AP

Synonyms

A15 homolog, T245, T245 protein, tetraspanin 6, Tetraspanin TM4 D, TM4SF6, TSPAN 6, TSPAN6

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using TSPAN6 Polyclonal antibody (12293-1-AP)

    WB analysis of mouse colon using 12293-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 12293-1-AP (TSPAN6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TSPAN6 Polyclonal antibody (12293-1-AP)

    WB analysis using 12293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12293-1-AP (TSPAN6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse colon tissue, rat colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12293-1-AP targets TSPAN6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSPAN6 fusion protein Ag2964 相同性解析による交差性が予測される生物種
Full Name tetraspanin 6
Calculated molecular weight 245 aa, 28 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC012389
Gene symbol TSPAN6
Gene ID (NCBI) 7105
RRIDAB_2213446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSPAN6 (also known as TM4SF6) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN6 gene is widely expressed, with high mRNA levels in liver, pancreas, kidney, and ovary (PMID: 9782095). TSPAN6 has been reported as a negative regulator of the RLR pathway by interacting with MAVS in a ubiquitination-dependent manner (PMID: 22908223).

Protocols

Product Specific Protocols
WB protocol for TSPAN6 antibody 12293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols