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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TSPO/PBR Monoclonal antibody

TSPO/PBR Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1F12

Cat no : 68137-1-Ig

Synonyms

TSPO, Peripheral-type benzodiazepine receptor, MBR, BZRP, 1D1F12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    WB analysis using 68137-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    WB analysis using 68137-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of Jurkat cells using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    WB analysis of Jurkat using 68137-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using TSPO/PBR Monoclonal antibody (68137-1-Ig)

    IF Staining of NIH/3T3 using 68137-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TSPO/PBR antibody (68137-1-Ig, Clone: 1D1F12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inK-562 cells, LNCaP cells, Jurkat cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68137-1-Ig targets TSPO/PBR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen TSPO/PBR fusion protein Ag7475 相同性解析による交差性が予測される生物種
Full Name translocator protein (18kDa)
Calculated molecular weight 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC001110
Gene symbol TSPO
Gene ID (NCBI) 706
RRIDAB_2923664
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSPO, also named Peripheral-type benzodiazepine receptor (PBR), encodes the translocator protein, belonging to tryptophan-rich sensory protein family. The protein is mainly localized in the outer mitochondrial membrane and expressed in steroid-synthesizing tissues (PMID:32881658, PMID:1326278). TSPO is involved in the translocation of cholesterol from the outer to inner mitochondrial membrane (PMID:21119734). In response to injury, TSPO has a upregulated expression in the peripheral nervous system (PMID:32423330, PMID:23251719). TSPO is also as a biomarker in a non-invasive procedure including detecting the inflammation in the heart and atherosclerotic plaques (PMID:24402571).

Protocols

Product Specific Protocols
WB protocol for TSPO/PBR antibody 68137-1-IgDownload protocol
IF protocol for TSPO/PBR antibody 68137-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols