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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TSPO/PBR Polyclonal antibody (15444-1-AP)

    WB analysis of HEK-293 using 15444-1-AP (same clone as 15444-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15444-1-AP (TSPO/PBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15444-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TSPO/PBR Polyclonal antibody (15444-1-AP)

    IHC staining of mouse heart using 15444-1-AP (same clone as 15444-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15444-1-AP (TSPO/PBR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15444-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using TSPO/PBR Polyclonal antibody (15444-1-AP)

    IHC staining of mouse heart using 15444-1-AP (same clone as 15444-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15444-1-AP (TSPO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15444-1-PBS in a different storage buffer formulation.

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TSPO/PBR Polyclonal antibody, PBS Only

TSPO/PBR Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15444-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15444-1-PBS targets TSPO/PBR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSPO/PBR fusion protein Ag7475 相同性解析による交差性が予測される生物種
Full Name translocator protein (18kDa)
Calculated molecular weight 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC001110
Gene Symbol TSPO
Gene ID (NCBI) 706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30536
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TSPO, also named Peripheral-type benzodiazepine receptor (PBR), encodes the translocator protein, belonging to tryptophan-rich sensory protein family. The protein is mainly localized in the outer mitochondrial membrane and expressed in steroid-synthesizing tissues (PMID:32881658, PMID:1326278). TSPO is involved in the translocation of cholesterol from the outer to inner mitochondrial membrane (PMID:21119734). In response to injury, TSPO has a upregulated expression in the peripheral nervous system (PMID:32423330, PMID:23251719). TSPO is also as a biomarker in a non-invasive procedure including detecting the inflammation in the heart and atherosclerotic plaques (PMID:24402571).