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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSR1 Polyclonal antibody (29957-1-AP)

    WB analysis using 29957-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29957-1-AP (TSR1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using TSR1 Polyclonal antibody (29957-1-AP)

    IF Staining of U-251 using 29957-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using TSR1 antibody (29957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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TSR1 Polyclonal antibody

TSR1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29957-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Pre-rRNA-processing protein TSR1 homolog, KIAA1401, FLJ10534

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, L02 cells, U-251 cells
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29957-1-AP targets TSR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSR1 fusion protein Ag31575 相同性解析による交差性が予測される生物種
Full Name TSR1, 20S rRNA accumulation, homolog (S. cerevisiae)
Calculated molecular weight92KD
Observed molecular weight 100 kDa
GenBank accession numberNM_018128
Gene Symbol TSR1
Gene ID (NCBI) 55720
RRIDAB_3086199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2NL82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TSR1 antibody 29957-1-APDownload protocol
IF protocol for TSR1 antibody 29957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols