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  • Featured Product
  • KD/KO Validated

TSSC4 Polyclonal antibody

TSSC4 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14531-1-AP

Synonyms

U5 small nuclear ribonucleoprotein TSSC4, Tumor-suppressing subchromosomal transferable fragment candidate gene 4 protein, Tumor-suppressing STF cDNA 4 protein, Protein TSSC4

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung tissue using TSSC4 Polyclonal antibody (14531-1-AP)

    IHC staining of human lung using 14531-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14531-1-AP (TSSC4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using TSSC4 Polyclonal antibody (14531-1-AP)

    IHC staining of human lung using 14531-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14531-1-AP (TSSC4 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using TSSC4 Polyclonal antibody (14531-1-AP)

    IHC staining of mouse lung using 14531-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14531-1-AP (TSSC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TSSC4 Polyclonal antibody (14531-1-AP)

    IF Staining of HeLa using 14531-1-AP

    Immunofluorescent analysis of Hela cells, using TSSC4 antibody 14531-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive IHC detected inhuman lung tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14531-1-AP targets TSSC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSSC4 fusion protein Ag6030 相同性解析による交差性が予測される生物種
Full Name tumor suppressing subtransferable candidate 4
Calculated molecular weight 34 kDa
Observed molecular weight35-45 kDa
GenBank accession numberBC050616
Gene symbol TSSC4
Gene ID (NCBI) 10078
RRIDAB_11232415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for TSSC4 antibody 14531-1-APDownload protocol
IF protocol for TSSC4 antibody 14531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation.

Authors - Klára Klimešová
  • KD Validated
View Article