Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HT-29 cells, COLO 320 cells, mouse testis tissue, mouse colon tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83401-4-RR targets TSSK4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | TSSK4 fusion protein Ag16205 相同性解析による交差性が予測される生物種 |
| Full Name | testis-specific serine kinase 4 |
| Calculated molecular weight | 338 aa, 37 kDa |
| Observed molecular weight | 38-45 kDa |
| GenBank accession number | BC111088 |
| Gene Symbol | TSSK4 |
| Gene ID (NCBI) | 283629 |
| RRID | AB_3671052 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6SA08 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSSK4 (Testis-specific serine/threonine-protein kinase 4), testis-specific serine/threonine protein kinase 4, is a member of the testis-specific serine/threonine protein kinase family. It has autophosphorylation activity and self-binding properties; it can interact with and phosphorylate other proteins, e.g., it can bind to and phosphorylate Odf2. It's reported that TSSK4 plays an important role in spermatogenesis, TSSK4 knockout male mice develop reduced sperm motility and reduced fertility. In addition, TSSK4 regulates lung fibrosis by promoting the loss of alveolar epithelial type II cells through the HSP90-AKT signaling pathway.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TSSK4 antibody 83401-4-RR | Download protocol |
| IHC protocol for TSSK4 antibody 83401-4-RR | Download protocol |
| IF protocol for TSSK4 antibody 83401-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |