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- KD/KO Validated
TTC19 Polyclonal antibody
TTC19 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 20875-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, HeLa cells, rat heart tissue |
| Positive IHC detected in | human breast cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 2 publications below |
Product Information
20875-1-AP targets TTC19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TTC19 fusion protein Ag14974 相同性解析による交差性が予測される生物種 |
| Full Name | tetratricopeptide repeat domain 19 |
| Calculated molecular weight | 501 aa, 56 kDa |
| Observed molecular weight | 32-35 kDa |
| GenBank accession number | BC011698 |
| Gene symbol | TTC19 |
| Gene ID (NCBI) | 54902 |
| RRID | AB_2878755 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TTC19 is a subunit of mitochondrial respiratory chain complex III and is proposed to preserve the structural and functional integrity of complex III. Loss-of-function mutations in TTC19 have been found in patients with complex III deficiency associated with heterogeneous neurological syndromes. The precursor TTC19 was detected as a 43 kDa cytoplasmic protein, while the mature TTC19 with the mitochondrial targeting sequence cleaved was observed as 35 kDa by SDS-PAGE. (PMID: 21278747)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TTC19 antibody 20875-1-AP | Download protocol |
| IHC protocol for TTC19 antibody 20875-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci XPOT Disruption Suppresses TNBC Growth through Inhibition of Specific tRNA Nuclear Exportation and TTC19 Expression to Induce Cytokinesis Failure
| ||
Elife Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes |
