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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTC21B Polyclonal antibody (30112-1-AP)

    WB analysis using 30112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30112-1-AP (TTC21B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TTC21B Polyclonal antibody (30112-1-AP)

    IHC staining of mouse testis using 30112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30112-1-AP (TTC21B antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

TTC21B Polyclonal antibody

TTC21B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30112-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

FLJ11457, IFT139, KIAA1992, Nbla10696, THM1, TPR repeat protein 21B, TTC21B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30112-1-AP targets TTC21B in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC21B fusion protein Ag32456 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 21B
Calculated molecular weight151KD
Observed molecular weight 55 kDa
GenBank accession numberNM_024753
Gene Symbol TTC21B
Gene ID (NCBI) 79809
RRIDAB_3086232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4L5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TTC21B, also known as THM1, IFT139, belongs to the TTC21 family, containing several tetratricopeptide repeat (TPR) domains. TTC21B is a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs). TTC21B is essential for retrograde trafficking of IFT-1, IFT-B, and GPCRs (PubMed:27932497). TTC21B is localized to the cilium axoneme and may play a role in retrograde intraflagellar transport in cilia (PMID: 18327258). Mutations in TTC21B are associated with nephronophthisis 12 (NPHP12)and short-rib thoracic dysplasia 4 with or without polydactyly (SRTD4)(PMID: 21258341).

Protocols

Product Specific Protocols
WB protocol for TTC21B antibody 30112-1-APDownload protocol
IHC protocol for TTC21B antibody 30112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols