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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TTC26 Polyclonal antibody

TTC26 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25083-1-AP

Synonyms

IFT56, dyf 13

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse testis tissue using TTC26 Polyclonal antibody (25083-1-AP)

    WB analysis of mouse testis using 25083-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25083-1-AP (TTC26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TTC26 Polyclonal antibody (25083-1-AP)

    WB analysis of HEK-293 using 25083-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25083-1-AP (TTC26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using TTC26 Polyclonal antibody (25083-1-AP)

    WB analysis of NIH/3T3 using 25083-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25083-1-AP (TTC26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using TTC26 Polyclonal antibody (25083-1-AP)

    IF Staining of hTERT-RPE1 using 25083-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using TTC26 antibody (25083-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Tested Applications

Positive WB detected inmouse testis tissue, HEK-293 cells, NIH/3T3 cells
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25083-1-AP targets TTC26 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC26 fusion protein Ag18983 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 26
Calculated molecular weight 554 aa, 64 kDa
Observed molecular weight 60-64 kDa
GenBank accession numberBC126331
Gene symbol TTC26
Gene ID (NCBI) 79989
RRIDAB_2651173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TTC26 (Tetratricopeptide repeat protein 26), also known as IFT56 (Intraflagellar transport protein 56), is an IFT complex B protein, which has a role in transport of ciliary cargo, important for ciliary motility and construction of full length cilia (PMID: 38135897). Severe biliary ciliopathy, caused by bi-allelic mutations in TTC26, has been recently described in the context of a syndrome of polydactyly and severe neonatal cholestasis, with brain, kidney and heart involvement (PMID: 32617964).

Protocols

Product Specific Protocols
WB protocol for TTC26 antibody 25083-1-APDownload protocol
IF protocol for TTC26 antibody 25083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Authors - Tomoharu Kanie
View Article