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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TTC37 Polyclonal antibody

TTC37 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24594-1-AP

Synonyms

TPR repeat protein 37, Thespin, Superkiller complex protein 3, SKIC3, Ski3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTC37 Polyclonal antibody (24594-1-AP)

    WB analysis using 24594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TTC37 Polyclonal antibody (24594-1-AP)

    WB analysis of MCF-7 using 24594-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TTC37 Polyclonal antibody (24594-1-AP)

    WB analysis of HeLa using 24594-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24594-1-AP (TTC37 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using TTC37 Polyclonal antibody (24594-1-AP)

    IP experiment of mouse testis using 24594-1-AP

    IP result of anti-TTC37 (IP:24594-1-AP, 4ug; Detection:24594-1-AP 1:300) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TTC37 Polyclonal antibody (24594-1-AP)

    IHC staining of human tonsillitis using 24594-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TTC37 Polyclonal antibody (24594-1-AP)

    IHC staining of human kidney using 24594-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TTC37 Polyclonal antibody (24594-1-AP)

    IHC staining of human kidney using 24594-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TTC37 Polyclonal antibody (24594-1-AP)

    IHC staining of human tonsillitis using 24594-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24594-1-AP (TTC37 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TTC37 Polyclonal antibody (24594-1-AP)

    IF Staining of HepG2 using 24594-1-AP

    Immunofluorescent analysis of HepG2 cells using 24594-1-AP (TTC37 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

24594-1-AP targets TTC37 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC37 fusion protein Ag18442 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 37
Calculated molecular weight 1564 aa, 175 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC056893
Gene symbol TTC37
Gene ID (NCBI) 9652
RRIDAB_2879628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TTC37 (tetratricopeptide repeat protein 37), also named as Thespin, encodes 1,564 amino acid protein that contains twenty tetratricopeptide (TPR) repeats. TPR repeat-containing motifs are found in a variety of proteins and may mediate protein-protein interactions and chaperone activity. Although not expressed in liver, TTC37 is a widely expressed protein with highest levels of expression in vascular tissues, lymph node, pituitary, lung and intestine. TTC37 is a component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C). Defects in TTC37 are associated with Trico-Hepato-Enteric (THE) syndrome.

Protocols

Product Specific Protocols
WB protocol for TTC37 antibody 24594-1-APDownload protocol
IHC protocol for TTC37 antibody 24594-1-APDownload protocol
IF protocol for TTC37 antibody 24594-1-APDownload protocol
IP protocol for TTC37 antibody 24594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Clin Invest

Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease.

Authors - Kun Yang
View Article
humanWB

PLoS Genet

A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies.

Authors - Masakazu Kohda
View Article